TOOLStripping Buffer
TOOLStripping Buffer is a ready-to-use buffer and it safely and effectively removes primary and secondary antibodies from western blot membranes. Simply incubate this buffer with PVDF or nitrocellulose membrane to allow Western blots to be re-probed with other antibodies.
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Ready to use reagent.
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Effectively removes primary and secondary antibodies from western blot membranes.
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3 min complete stripping procedure.
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No stinky smelling (without addition of 2-ME)
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Non-hazardous solution
Fig 1.
Lane 1: marker, Lane 2: RD cells, Lane 3: Stau1-out RD cells
Target protein: Stau1
Protein input: 30ug
Exposure time: 600S'
1° antibody dilution 1:1000
2° antibody dilution 1:5000
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Chang JH, Cheng CW, Yang YC, Chen WS, Hung WY, Chow JM, Chen PS, Hsiao M, Lee WJ, Chien MH. Downregulating CD26/DPPIV by apigenin modulates the interplay between Akt and Snail/Slug signaling to restrain metastasis of lung cancer with multiple EGFR statuses. J Exp Clin Cancer Res. 2018 Aug 22;37(1):199.
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Lin YH, Wu MH, Huang YH, Yeh CT, Chi HC, Tsai CY, Chuang WY, Yu CJ, Chung IH, Chen CY, Lin KH. Thyroid hormone negatively regulates tumorigenesis through suppression of BC200. Endocr Relat Cancer. 2018 Dec 1;25(12):967-979.
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Wu YY, Hsieh CT, Chiu YM, Chou SC, Kao JT, Shieh DC, Lee YJ. GSK-3 inhibitors enhance TRAIL-mediated apoptosis in human gastric adenocarcinoma cells. PLoS One. 2018 Dec 17;13(12):e0208094.